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Field protocol eDNA

The field protocol consists of five steps, beginning with yearly monitoring of all waterways, which are divided in 5 km routes (Step 1). In the event that a route tests positive for muskrat eDNA, more accurate localisation is performed by sampling every 1 km and every 100 m (Step 2). The eDNA results of Step 2 enable more accurate placement of the traps (Step 3). After a (successful) catching period, sequential check-ups are performed surrounding each catch/eDNA location, in order to confirm muskrats are no longer present at the catch location and no expulsion took place (Step 4 and 5).

Should a sample still test positive, Steps 3-5 are repeated until muskrat eDNA is no longer detected. The area under investigation is then declared free of muskrats, and will be tested again during regular monitoring.

Alternative starting point: In the event of civilian notification, the exterminator will investigate the location for the presence of muskrats. If traces are found, the field protocol is started at Step 2.


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