Field protocol for eDNA in use

In the research project eDNA, a field protocol for muskrat control has been drawn up.

  • The field protocol consists of five steps, beginning with yearly monitoring of all waterways, which are divided in 5 km routes (Step 1).
  • In the event that a route tests positive for muskrat eDNA, more accurate localisation is performed by sampling every 1 km and every 100 m (Step 2).
  • The eDNA results of Step 2 enable more accurate placement of the traps (Step 3).
  • After a (successful) catching period, sequential check-ups are performed surrounding each catch/eDNA location, in order to confirm muskrats are no longer present at the catch location and no expulsion took place (Step 4 and 5).

Free of muskrats

Should a sample still test positive, Steps 3-5 are repeated until muskrat eDNA is no longer detected. The area under investigation is then declared free of muskrats, and will be tested again during regular monitoring.

Civilian notification

Alternative starting point: In the event of civilian notification, the exterminator will investigate the location for the presence of muskrats. If traces are found, the field protocol is started at Step 2.

Download field protocol